Enzymatic detoxification of lignocellulose pulping solutions

REM image of an EC-EA carrier particle after a laccase-catalyzed reaction of an organosolv digestion solution with 20 mM phenolic components.
SEM image of an EC-EA carrier particle after a laccase-catalyzed reaction of an organosolv digestion solution with 20 mM phenolic components.

The lignocellulose pulping solution may contain toxic degradation products such as furfural or hydroxymethyl-furfural, which can impair the growth of microorganisms.

Fraunhofer IGB has established a process for detoxification with an immobilized laccase of Trametes versicolor for the removal of growth-inhibiting substances. Through laccasecatalyzed polymerization and precipitation, interfering aromatics can be easily removed by filtration. A minimum enzyme concentration was determined that is sufficient to remove more than 80 percent of the aromatic substances from the solution. The remaining toxic substances can then be removed from the fractions, e.g. by adsorption on carrier materials.

The digestion solution rich in hemicelluloses can then be used in fermentations with certain microorganisms. This is also part of the work at Fraunhofer IGB.

Publications

Ludwig D, Amann M, Hirth T, Rupp S, Zibek S (2013) Development and optimization of single and combined detoxification processes to improve the fermentability of lignocellulose hydrolyzates. Bioresource Technology, vol 133. doi:http://dx.doi.org/10.1016/j.biortech.2013.01.053

Reference projects

Lignocellulose biorefinery

 

The aim was to develop and establish a process for the complete material utilization of all lignocellulose components by obtaining biobased products based on cellulose, hemicellulose and lignin within a biorefinery.

 

Duration: January 2010 – December 2013